자연모사소재공정연구소(에너지과학과)에서 Nathaniel S. Hwang 교수님을 모시고 세미나를 개최하오니 관심 있는 분들의 많은 참석 부탁드립니다.

Mar. 26th , 2019, 16:00~16:50 PM
Room No. 86120 (N Center), Sungkyunkwan University, Suwon

Synthetic Inorganic Nanoparticles Reinforced Cryogel for Bone Regeneration

Prof. Nathaniel S. Hwang
(School of Chemical and Biological Engineering, Seoul National University
Interdisciplinary Program in Bioengineering, Seoul National University)


Abstract
Bone is a vascularized tissue that is comprised of collagen fibers and calcium phosphate crystals such as hydroxyapatite (HAp) and whitlockite (WH). HAp and WH are known to elicit bone regeneration by stimulating osteoblast activities and osteogenic commitment of stem cells. In addition, vascular endothelial growth factor (VEGF) is shown to promote osteogenesis and angiogenesis which is considered as an essential process in bone repair by providing nutrients. In this study, VEGF‐secreting human adipose‐derived stem cells (VEGF‐ADSCs) are developed by transducing ADSCs with VEGF‐encoded lentivirus. Additionally, WH‐reinforced gelatin/heparin cryogels (WH‐C) are fabricated by loading WH into gelatin/heparin cryogels. VEGF‐ADSC secrete tenfold more VEGF than ADSC and show increased VEGF secretion with cell growth. Also, incorporation of WH into cryogels provides a mineralized environment with ions secreted from WH. When the VEGF‐ADSCs are seeded on WH‐C, sustained release of VEGF is observed due to the specific affinity of VEGF to heparin. Finally, the synergistic effect of VEGF‐ADSC and WH on osteogenesis is successfully confirmed by alkaline phosphatase and real‐time polymerase chain reaction analysis. In vivo bone formation is demonstrated via implantation of VEGF‐ADSC seeded WH‐C into mouse calvarial bone defect model, resulted in enhanced bone development with the highest bone volume/total volume.